EEG-based auditory attention decoding with audiovisual speech for hearing-impaired listeners.

Auditory attention decoding (AAD) was used to determine the attended speaker during an auditory selective attention task. However, the auditory factors modulating AAD remained unclear for hearing-impaired (HI) listeners. In this study, scalp electroencephalogram (EEG) was recorded with an auditory selective attention paradigm, in which HI listeners were instructed to attend one of the two simultaneous speech streams with or without congruent visual input (articulation movements), and at a high or low target-to-masker ratio (TMR). Meanwhile, behavioral hearing tests (i.e. audiogram, speech reception threshold, temporal modulation transfer function) were used to assess listeners' individual auditory abilities. The results showed that both visual input and increasing TMR could significantly enhance the cortical tracking of the attended speech and AAD accuracy. Further analysis revealed that the audiovisual (AV) gain in attended speech cortical tracking was significantly correlated with listeners' auditory amplitude modulation (AM) sensitivity, and the TMR gain in attended speech cortical tracking was significantly correlated with listeners' hearing thresholds. Temporal response function analysis revealed that subjects with higher AM sensitivity demonstrated more AV gain over the right occipitotemporal and bilateral frontocentral scalp electrodes.

The Mandarin Chinese auditory emotions stimulus database: A validated set of Chinese pseudo-sentences.

Emotional prosody is fully embedded in language and can be influenced by the linguistic properties of a specific language. Considering the limitations of existing Chinese auditory stimulus database studies, we developed and validated an emotional auditory stimuli database composed of Chinese pseudo-sentences, recorded by six professional actors in Mandarin Chinese. Emotional expressions included happiness, sadness, anger, fear, disgust, pleasant surprise, and neutrality. All emotional categories were vocalized into two types of sentence patterns, declarative and interrogative. In addition, all emotional pseudo-sentences, except for neutral, were vocalized at two levels of emotional intensity: normal and strong. Each recording was validated with 40 native Chinese listeners in terms of the recognition accuracy of the intended emotion portrayal; finally, 4361 pseudo-sentence stimuli were included in the database. Validation of the database using a forced-choice recognition paradigm revealed high rates of emotional recognition accuracy. The detailed acoustic attributes of vocalization were provided and connected to the emotion recognition rates. This corpus could be a valuable resource for researchers and clinicians to explore the behavioral and neural mechanisms underlying emotion processing of the general population and emotional disturbances in neurological, psychiatric, and developmental disorders. The Mandarin Chinese auditory emotion stimulus database is available at the Open Science Framework ( https://osf.io/sfbm6/?view_only=e22a521e2a7d44c6b3343e11b88f39e3 ).

The Associations between Sleep Duration, Academic Pressure, and Depressive Symptoms among Chinese Adolescents: Results from China Family Panel Studies.

Depressive symptoms are a common mental health problem among adolescents, which may affect their physical and mental health development and impose heavy burdens on individual families and society. This study aimed to examine the associations between sleep duration, academic pressure, and depressive symptoms among Chinese adolescents and to construct the mediation model to explore the mediating effect of sleep duration. The data are from the China Family Panel Studies (CFPS). Methodologically, the aforementioned associations were explored by constructing a structural equation model and applying multivariate multilevel logistic regression. In this study, we found that approximately 6.49% of the 3724 Chinese adolescents had depressive symptoms. Sleep duration of <6 h/night (OR = 2.39, 95%CI = 1.33-4.32) and high/maximum academic pressure (high: OR = 1.43, 95%CI = 1.02-1.99; maximum: OR = 2.43, 95%CI = 1.58-3.73) were both associated with an increased risk of depressive symptoms in adolescents. Meanwhile, the multiplicative interaction between sleep duration and academic pressure was significantly associated with depressive symptoms in adolescents (p < 0.001). The sleep duration played a partial mediating role in the relationship between academic pressure and depressive symptoms (a*b = 0.006, 95%BootCI = 0.001-0.012). Our study highlights that it is essential to mitigate the academic pressure of adolescents to increase their sleep duration and further reduce the occurrence of depressive symptoms by adopting corresponding preventive measures.

Trajectory patterns of blood pressure change up to six years and the risk of dementia: a nationwide cohort study.

The present study aimed to investigate the associations between the trajectory of blood pressure (BP) change and the risk of subsequent dementia and to explore the differences in age, gender, and hypertension subgroups. We included 10,660 participants aged ≥ 60 years from 1998 to 2018 waves of the Chinese Longitudinal Healthy Longevity Survey. Latent growth mixture models were used to estimate BP trajectories. Cox-proportional hazard models were used to analyze the effects of BP trajectories on the risk of dementia. According to the results, stabilized systolic BP (SBP) was found to be associated with a higher risk of dementia compared with normal SBP [adjusted hazard ratio (aHR): 1.62; 95% confidence interval (CI): 1.27-2.07] and elevated SBP (aHR: 2.22; 95% CI: 1.51-3.28) in and only in the subgroups of the oldest-old, women, and subjects without hypertension at baseline. Similarly, stabilized pulse pressure (PP) was associated with a higher risk of dementia compared with normal PP (aHR: 1.52; 95% CI: 1.24-1.88) and elevated PP (aHR: 2.12; 95% CI: 1.48-3.04) in and only in the subgroups of the oldest-old, women, and subjects with hypertension at baseline. These findings suggest that stabilized SBP and PP have predictive significance for the occurrence of dementia in late life, and the factors of age, gender, and late-life hypertension should be considered when estimating the risk of BP decline on dementia.

Categorical perception of lexical tones based on acoustic-electric stimulation.

The effect of low-frequency acoustic input on the categorical perception of lexical tones was investigated with simulated electric-acoustic hearing. A synthesized T1-T2 (flat-rising) tone continuum of Mandarin monosyllables /i/ was used, and they were manipulated as five conditions: unprocessed, low-frequency acoustic-only, electric-only, electric-acoustic stimulation, and bimodal stimulation. Results showed the performance under electric-only condition was the significantly lowest, and the difference of other pairwise comparisons between conditions was quite small. These findings suggest that the low-frequency acoustic input can shape the categorical perception, and the combinations of acoustic and electric hearing within or across ears have no significant effect.

The Effect of Parenting Quality on Child Development at 36-48 Months in China's Urban Area: Evidence from a Birth Cohort Study.

Environmental exposures, especially parenting quality, are critical for later child development. This study aimed to determine the status of parenting quality and suspected development delay of preschool children in China's urban area and explore the associations between these two factors. The research was based on a birth cohort study conducted in Changsha, Hunan province, China. We used the Parenting Assessment Tool and Ages and Stages Questionnaires, Third Edition (ASQ-3), to measure parenting quality and child development status, respectively. Other data were collected from maternal health manuals and self-administered questionnaires during the follow-up period. The generalized estimating equation was used to examine whether parenting quality was significantly associated with child development outcomes. In the study, good parenting quality was 33.6% measured at 18 months, and suspected development delay was below 10% at 36-48 months among urban China; we observed negative associations between parenting quality scores and child development scores; poor parenting quality had a negative association with suspected development delay [OR and 95% CI: 2.74 (1.17, 6.40)], girls [OR and 95% CI: 0.33 (0.16, 0.69)] and maternal education years (>12 years) [OR and 95% CI: 0.27 (0.12, 0.64)] were protective factors for suspected development delay. Our findings highlighted the importance of good parenting quality among children in urban areas of China through a birth cohort study and may be used to reduce the children at high risk of developmental delay as a future intervention program.

UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa.

To elucidate potential roles of UDP-glucose pyrophosphorylase (UGP) in mycelial growth and polysaccharide synthesis of Grifola frondosa, a putative 2036-bp UDP-glucose pyrophosphorylase gene gfugp encoding a 53.17-kDa protein was cloned and re-annotated. Two dual promoter RNA silencing vectors of pAN7-iUGP-P-dual and pAN7-iUGP-C-dual were constructed to down-regulate gfugp expression by targeting its promoter or conserved functional sequences, respectively. Results showed that silence of gfugp promoter sequence had a higher down-regulating efficiency with slower mycelial growth and polysaccharide production than those of conserved sequence. The monosaccharide compositions/percentages of mycelial and exo-polysaccharides significantly changed with the increase of galactose and arabinose contents possibly due to block of UDP-glucose supply by gfugp silence and alteration of sugar metabolism via up-regulation of UDP-glucose-4-epimerase (gfuge) and UDP-xylose-4-epimerase (gfuxe) transcription. Our findings would provide a reference to know the biosynthesis pathway of mushroom polysaccharides and improve their production by metabolic regulation.

The Associations between the Duration of Folic Acid Supplementation, Gestational Diabetes Mellitus, and Adverse Birth Outcomes based on a Birth Cohort.

This study aimed to examine the associations between the duration of folic acid (FA) supplementation, gestational diabetes mellitus (GDM), and adverse birth outcomes. A total of 950 mother-offspring pairs participated in the cohort study during 2015 in Changsha, China. The data were collected through home visits and perfected by maternal and child healthcare handbooks. Generalized linear models and stratified analyses were used for statistical analyses. The incidence of GDM in our cohort was 10.2%. FA supplementation for ≥3 months before pregnancy was associated with an increased risk of GDM (adjusted relative risk (aRR): 1.72; 95% CI: 1.17-2.53) and decreased risk of small-for-gestational-age (SGA) birth (aRR: 0.40; 95% CI: 0.18-0.88). In the group of FA supplementation for ≥3 months during pregnancy, GDM was associated with an increased risk of cesarean delivery (aRR: 1.36; 95% CI: 1.06-1.75) and macrosomia (aRR: 2.11; 95% CI: 1.06, 4.20), but the aRRs were lower than the RRMH 1.53 (95% CI: 1.01-2.34) and 2.43 (95% CI: 1.27-4.66). Our study suggested that the longer duration of FA supplementation before pregnancy might increase the risk of GDM, but decrease the risk of SGA birth. Longer duration of FA supplementation during pregnancy had beneficial effects on birth outcomes in women with GDM. Further studies should consider a larger sample size to confirm these findings.

Congruent audiovisual speech enhances auditory attention decoding with EEG.

OBJECTIVE: The auditory attention decoding (AAD) approach can be used to determine the identity of the attended speaker during an auditory selective attention task, by analyzing measurements of electroencephalography (EEG) data. The AAD approach has the potential to guide the design of speech enhancement algorithms in hearing aids, i.e. to identify the speech stream of listener's interest so that hearing aids algorithms can amplify the target speech and attenuate other distracting sounds. This would consequently result in improved speech understanding and communication and reduced cognitive load, etc. The present work aimed to investigate whether additional visual input (i.e. lipreading) would enhance the AAD performance for normal-hearing listeners. APPROACH: In a two-talker scenario, where auditory stimuli of audiobooks narrated by two speakers were presented, multi-channel EEG signals were recorded while participants were selectively attending to one speaker and ignoring the other one. Speakers' mouth movements were recorded during narrating for providing visual stimuli. Stimulus conditions included audio-only, visual input congruent with either (i.e. attended or unattended) speaker, and visual input incongruent with either speaker. The AAD approach was performed separately for each condition to evaluate the effect of additional visual input on AAD. MAIN RESULTS: Relative to the audio-only condition, the AAD performance was found improved by visual input only when it was congruent with the attended speech stream, and the improvement was about 14 percentage points on decoding accuracy. Cortical envelope tracking activities in both auditory and visual cortex were demonstrated stronger for the congruent audiovisual speech condition than other conditions. In addition, a higher AAD robustness was revealed for the congruent audiovisual condition, with reduced channel number and trial duration achieving higher accuracy than the audio-only condition. SIGNIFICANCE: The present work complements previous studies and further manifests the feasibility of the AAD-guided design of hearing aids in daily face-to-face conversations. The present work also has a directive significance for designing a low-density EEG setup for the AAD approach.

The effect of speech material on the band importance function for Mandarin Chinese.

Speech material influences the relative contributions of different frequency regions to intelligibility for English. In the current study, whether a similar effect of speech material is present for Mandarin Chinese was investigated. Speech recognition was measured using three speech materials in Mandarin, including disyllabic words, nonsense sentences, and meaningful sentences. These materials differed from one another in terms of the amount of contextual information and word frequency. The band importance function (BIF), as defined under the Speech Intelligibility Index (SII) framework, was used to quantify the contributions across frequency regions. The BIFs for the three speech materials were estimated from 16 adults who were native speakers of Mandarin. A Bayesian adaptive procedure was used to efficiently estimate the octave-frequency BIFs for the three materials for each listener. As the amount of contextual information increased, low-frequency bands (e.g., 250 and 500 Hz) became more important for speech recognition, consistent with English. The BIF was flatter for Mandarin than for comparable English speech materials. Introducing the language- and material-specific BIFs to the SII model led to improved predictions of Mandarin speech-recognition performance. Results suggested the necessity of developing material-specific BIFs for Mandarin.

Brainstem encoding of frequency-modulated sweeps is relevant to Mandarin concurrent-vowels identification for normal-hearing and hearing-impaired listeners.

F0 contours convey the primary information of lexical Tones for Mandarin Chinese, and the processing of time-varying F0 contours is important for Mandarin concurrent-vowels identification (MCVI). In this work, we examined the relationship between frequency modulation (FM) detection of auditory system and MCVI in both normal-hearing (NH) and hearing-impaired (HI) listeners. Three experiments were conducted with the same subjects to measure their MCVI, FM detection limen (FMDL), and frequency following responses (FFRs) evoked by FM sweeps, respectively. To ensure that F0 contour was the primary cue utilized, mean F0s and durations were equalized among all test vowels in the MCVI experiment. To simulate the pattern of F0 contours of Mandarin vowels, linearly FM sweeps were used as stimuli in the FMDL and FFRs experiments. The results confirmed that the performance of HI listeners was significantly worse than that of NH listeners in all of the three measurements. Besides, FFRs evoked by FM sweeps had significantly lower tracking accuracy than those evoked by steady tones only for HI listeners. The correlation analysis further revealed that any two of the three measured indices were significantly correlated when the effects of age and absolute threshold were partialed out (|r| ≥ 0.502, p ≤ 0.017). These results suggested an association between the poor performance of HI listeners in the MCVI task and their degraded auditory function on FM detection, and such a behavioral degradation has emerged in the phase-locking activity at the brainstem level.

Functions of a Glucan Synthase Gene GFGLS in Mycelial Growth and Polysaccharide Production of Grifola frondosa.

Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation, and growth. In the present study, a glucan synthase gene (GFGLS) in the edible mushroom Grifola frondosa with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp is a membrane protein containing two large transmembrane domains connected with a hydrophilic cytoplasmic domain. With a constructed dual promoter RNA silencing vector pAN7-gfgls-dual, a GFGLS-silencing transformant iGFGLS-3 had the lowest GFGLS transcriptional expression level (26.1%) with a shorter length and thinner appearance of the mycelia, as well as decreased mycelial biomass and exo-polysaccharide production of 5.02 and 0.38 g/L, respectively. Further analysis indicated that GFGLS silence influenced slightly the monosaccharide compositions and ratios of mycelial and exo-polysaccharide. These findings suggest that GFGLS could affect mycelial growth and polysaccharide production by downregulating the glucan synthesis.

Differences between WHO Growth Standards and China Growth Standards in Assessing the Nutritional Status of Children Aged 0-36 Months Old.

Background: At present, whether to use the World Health Organization's (WHO) growth standards or native growth standards to assess the nutritional status in a given population is unclear. This study aimed to compare the differences between the WHO's growth standards and China's growth standards in assessing the nutritional status of children aged 0~36 months. Methods: We used z-scores to evaluate the nutritional status of children. The weight-for-age z-scores (WAZs), length/height-for-age z-scores (LAZ/HAZs), and weight-for-length/height z-scores (WLZ/WHZs) were calculated using the WHO's growth standards and China's growth standards. MeNemar's test was used to compare the nutritional status of children. Results: The results in this study showed that there were differences between the WHO's standards and China's standards in assessing children's nutritional status except for stunting and obesity. The prevalence of underweight assessed using China's standards was higher than when using the WHO's standards (except when 3 and 36 months old). The prevalence of wasting was significantly higher when assessed using China's standards than when using the WHO's standards from 12 to 36 months. The prevalence of overweight was higher when assessed using the WHO's standards from 3 to 8 months. Conclusions: Both the WHO's and China's growth standards are useful measures in assessing children's nutritional status but with key significant differences. Therefore, caution should be taken in selecting appropriate measures in a given population.

Speaking rhythmically improves speech recognition under "cocktail-party" conditions.

This study examines whether speech rhythm affects speech recognition under "cocktail-party" conditions. Against a two-talker masker, but not a speech-spectrum noise masker, recognition of the last (third) keyword in a normal rhythmic sentence was significantly better than that of the first keyword. However, this word-position-related speech-recognition improvement disappeared for rhythmically hybrid target sentences that were constructed by grouping parts from different sentences with different artificially modulated rhythms (rates) (fast, normal, or slow). Thus, the normal rhythm with a constant rate plays a role in improving speech recognition against informational speech masking, probably through a build-up of temporal prediction for target words.

The effect of F0 contour on the intelligibility of speech in the presence of interfering sounds for Mandarin Chinese.

In Mandarin Chinese, the fundamental frequency (F0) contour defines lexical "Tones" that differ in meaning despite being phonetically identical. Flattening the F0 contour impairs the intelligibility of Mandarin Chinese in background sounds. This might occur because the flattening introduces misleading lexical information. To avoid this effect, two types of speech were used: single-Tone speech contained Tones 1 and 0 only, which have a flat F0 contour; multi-Tone speech contained all Tones and had a varying F0 contour. The intelligibility of speech in steady noise was slightly better for single-Tone speech than for multi-Tone speech. The intelligibility of speech in a two-talker masker, with the difference in mean F0 between the target and masker matched across conditions, was worse for the multi-Tone target in the multi-Tone masker than for any other combination of target and masker, probably because informational masking was maximal for this combination. The introduction of a perceived spatial separation between the target and masker, via the precedence effect, led to better performance for all target-masker combinations, especially the multi-Tone target in the multi-Tone masker. In summary, a flat F0 contour does not reduce the intelligibility of Mandarin Chinese when the introduction of misleading lexical cues is avoided.

Individually tailored spectral-change enhancement for the hearing impaired.

An algorithm for enhancing spectral changes over time was previously shown to improve the intelligibility of speech in steady speech-spectrum noise (SSN) for hearing-impaired subjects but tended to impair intelligibility for speech in a background of two-talker speech. Large individual differences were found and the application of a genetic algorithm for selecting the "best" parameter values for each listener was found to be beneficial. In the present study, the spectral-change enhancement (SCE) processing was modified by individually tailoring the degree of SCE based on the frequency-dependent hearing loss of the subjects, and by using finer frequency resolution. The effect of the modified SCE processing on the intelligibility and quality of speech in SSN and babble noise (BBN) was evaluated. Ten subjects with mild to moderate hearing loss were tested twice for all tests. The SCE processing led to small but significant improvements in the intelligibility of speech in both SSN and BBN, while the effect of the SCE processing on speech quality was small.

Emotionally conditioning the target-speech voice enhances recognition of the target speech under "cocktail-party" listening conditions.

Under a noisy "cocktail-party" listening condition with multiple people talking, listeners can use various perceptual/cognitive unmasking cues to improve recognition of the target speech against informational speech-on-speech masking. One potential unmasking cue is the emotion expressed in a speech voice, by means of certain acoustical features. However, it was unclear whether emotionally conditioning a target-speech voice that has none of the typical acoustical features of emotions (i.e., an emotionally neutral voice) can be used by listeners for enhancing target-speech recognition under speech-on-speech masking conditions. In this study we examined the recognition of target speech against a two-talker speech masker both before and after the emotionally neutral target voice was paired with a loud female screaming sound that has a marked negative emotional valence. The results showed that recognition of the target speech (especially the first keyword in a target sentence) was significantly improved by emotionally conditioning the target speaker's voice. Moreover, the emotional unmasking effect was independent of the unmasking effect of the perceived spatial separation between the target speech and the masker. Also, (skin conductance) electrodermal responses became stronger after emotional learning when the target speech and masker were perceptually co-located, suggesting an increase of listening efforts when the target speech was informationally masked. These results indicate that emotionally conditioning the target speaker's voice does not change the acoustical parameters of the target-speech stimuli, but the emotionally conditioned vocal features can be used as cues for unmasking target speech.

Expression and imprinting of DIO3 and DIO3OS genes in Holstein cattle.

DIO3 and DIO3OS are two imprinted genes identified in mouse and humans. The DIO3 gene, which encodes for the type 3 deiodinase, is preferentially expressed from the paternal allele, while the DIO3OS transcript is transcribed in opposite orientation to DIO3, multiple noncoding and alternatively splicing isoforms from maternal allele. In this study, the five splice variants of DIO3OS were identified in Holstein cattle and had complex, tissue-specific expression patterns observed in eight tissues, including heart, liver, spleen, lung, kidney, muscle, fat and brain. In the G+C rich region, upstream from the cattle DIO3 gene, there were three small conserved regions and some promoter elements similar to those observed in mouse and humans. An allele-specific expression analysis-based SNP method revealed that DIO3 and DIO3OS genes exhibited monoallelic expression in the eight tissues, indicating that DIO3 and DIO3OS are imprinted in cattle.

Selective Attention Enhances Beta-Band Cortical Oscillation to Speech under "Cocktail-Party" Listening Conditions.

Human listeners are able to selectively attend to target speech in a noisy environment with multiple-people talking. Using recordings of scalp electroencephalogram (EEG), this study investigated how selective attention facilitates the cortical representation of target speech under a simulated "cocktail-party" listening condition with speech-on-speech masking. The result shows that the cortical representation of target-speech signals under the multiple-people talking condition was specifically improved by selective attention relative to the non-selective-attention listening condition, and the beta-band activity was most strongly modulated by selective attention. Moreover, measured with the Granger Causality value, selective attention to the single target speech in the mixed-speech complex enhanced the following four causal connectivities for the beta-band oscillation: the ones (1) from site FT7 to the right motor area, (2) from the left frontal area to the right motor area, (3) from the central frontal area to the right motor area, and (4) from the central frontal area to the right frontal area. However, the selective-attention-induced change in beta-band causal connectivity from the central frontal area to the right motor area, but not other beta-band causal connectivities, was significantly correlated with the selective-attention-induced change in the cortical beta-band representation of target speech. These findings suggest that under the "cocktail-party" listening condition, the beta-band oscillation in EEGs to target speech is specifically facilitated by selective attention to the target speech that is embedded in the mixed-speech complex. The selective attention-induced unmasking of target speech may be associated with the improved beta-band functional connectivity from the central frontal area to the right motor area, suggesting a top-down attentional modulation of the speech-motor process.

Attentional modulation of informational masking on early cortical representations of speech signals.

To recognize speech in a noisy auditory scene, listeners need to perceptually segregate the target talker's voice from other competing sounds (stream segregation). A number of studies have suggested that the attentional demands placed on listeners increase as the acoustic properties and informational content of the competing sounds become more similar to that of the target voice. Hence we would expect attentional demands to be considerably greater when speech is masked by speech than when it is masked by steady-state noise. To investigate the role of attentional mechanisms in the unmasking of speech sounds, event-related potentials (ERPs) were recorded to a syllable masked by noise or competing speech under both active (the participant was asked to respond when the syllable was presented) or passive (no response was required) listening conditions. The results showed that the long-latency auditory response to a syllable (/bi/), presented at different signal-to-masker ratios (SMRs), was similar in both passive and active listening conditions, when the masker was a steady-state noise. In contrast, a switch from the passive listening condition to the active one, when the masker was two-talker speech, significantly enhanced the ERPs to the syllable. These results support the hypothesis that the need to engage attentional mechanisms in aid of scene analysis increases as the similarity (both acoustic and informational) between the target speech and the competing background sounds increases.